Informatics Publishing Limited

Urwashi D. Ramteke ¹, S. P. Wate ¹

Affiliations
1 Sharad Pawar College of Pharmacy, Wanadongari, Nagpur(M.S.)

Abstract

In this study, reverse phase high performance liquid chromatographic method has been developed and validated for the simultaneous determination of Levocetirizine and Phenylephrine in tablet dosage formulation. HPLC separation was achieved with Serveyor PDA/RI/FL model having Chromoquest 5 Software with 20.0 μl fixed loop injector, plus C18 (250mm×4.6mm, 5μm) column and UV detector as stationary phase and 10mM phosphate buffer (pH adjustedto 5.5) (80:20, v/v) as eluent, at a flow rate of 1.0ml/min. UV detection was performed at 278 nm. The retention time of Levocetirizine and Phenylephrine were found to 4.921 and 2.721min respectively. Results of the analysis were validated statistically and by recovery studies. The stability of Levocetirizine was maintained by carrying out all the operations in amber coloured glass wares with minimal exposure to light. The result of the studies showed that the proposed RP-HPLC method is simple, rapid, precise and accurate, which can be used for the routine determination of Levocetirizine and Phenylephrine in bulk and in its pharmaceutical dosage forms.

Keywords

Phenylephrine, Levocetirizine, HPLC, Validation

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R. S. Jumle ¹, A. S. Mundhey ¹, S. P. Wate ¹, S. S. Dangare ¹, U. D. Ramteke ¹

Affiliations
1 Department of Pharmaceutical Chemistry, Sharad Pawar College of Pharmacy, Nagpur, IN

Abstract

The present study deals with UV spectrophotometric method development and validation for estimation of Tizanidine and Aceclofenac tablet dosage form by Viedort's method and first order UV derivative spectrophotometry. The Vierodt's method involves measurement of absorbance at max of Tizaridine and Aceclofenac at 282 nm respectively. The linearity of Tizanidine and Aceclofenac was found to be in the range of 1-10 μg/ml respectively. The % recovery of Tizanidine and Aceclofenac was found out to be 99.2% and 99.69%s respectively. First order CV derivative spectrophotonmetry (D1 method). the zero crossing method was chosen as Tizanidine could he easily analyzed without any interference from Aceclofenac and vice-versa. Tizanidine was determined by measurement of its D1 amplitude at the zero crossing point of Aceclofenac at (270nm), While Aceclofenac was determined by measurement of its D1 amplitude at zero crossing point of Tizanidine at (318 nm) The proposed method was validated as per ICH guidelines.

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